Glycanase-Catalyzed Synthesis of Non-natural Oligosaccharides

Abstract

A new methodology for the preparation of non-natural oligosaccharides has been developed using various glycosyl fluorides as activated glycosyl donors for enzymatic polycondensation. The reaction proceeds in a complete regio- and stereoselective manner to afford oligosaccharides of well-defined structures. The resulting oligosaccharides are difficult to prepare by conventional chemical methods with use of protecting groups.