Abstract
Investigation of the biological functions of glycoconju-gates based on specific molecular interactions is an emerging area of great potential interest. With the advent of Biomolecular Interaction Analysis (BIA) Technology seven years ago, the BIACORE biosensor instrument has been extensively used to elucidate and characterize many biomolecular interactions which are well documented in the literature. In BIA technology, the biomolecule of interest is immobilized onto a sensor surface and a binding partner can then be passed over it in a mobile aqueous phase. Their interaction on the sensor surface can subsequently be monitored in real time without the use of labels. In this paper, applications of the BIACORE instrument as a research tool for investigating specific interactions of glycoconjugates is introduced, where binding kinetics were determined using Concanavalin A and immobilized asialofetuin in a model system.
