Assays for Glycosyltransferases

Abstract

A variety of methods for assaying glycosyltransferase activity have been developed driven by the specific interests and type of information required by researchers. These methods include the routine monitoring of activity during and after enzyme purification and in biocatalysis, detailed mechanistic and inhibition evaluations, and high throughput screening for novel glycosyltransferase activities or inhibitors. Representative assay methods for all of these applications including their advantages and limitations are summarized in this review.