2002 Volume 14 Issue 75 Pages 1-11
There are two strategies for the enzymatic synthesis of oligosaccharides; the use of glycosidases and the use of trans-ferases. The reaction using glycosidases can be further classified into two methods; the transglycosylation and the reverse hydrolysis reaction. In this review, I would like to summarize and discuss these by focusing on the regioselectivity in the synthesis of oligosaccharides by the transglycosylation and reverse hydrolysis reaction.