Abstract
Although Nuclear Magnetic Resonance (NMR) is one of the most powerful methods for determining molecular conformations, it was hard to apply it to protein-carbohydrate interaction conformational studies due to weak binding affinities. This problem has been solved by three dimensional NOESY-HSQC NMR techniques using synthesized 13C-labelled carbohydrate ligands. In addition, the liquid crystalline phase NMR, which is a new and revolutionary NMR method for the analysis of proteins, has been successfully applied to protein-carbohydrate interaction research field and has been able to give more highly sensitive results.
