Abstract
Application of β-fructofuranosidase from Arthrobacter sp. K-1 to production of “functional oligosaccharides” including xylsucrose, isomaltosucrose and lactosucrose, and fructosylstevioside via transfructosylation was described. Xylsucrose and isomaltosucrose are non-cariogenic saccharides. Lactosucrose is non-digestive and has strong bifidus growth activity. The fructofuranosidase selectively transfers a fructosyl residue to the glucosyl residue at the 19-carboxyl group of stevioside. The quality of taste, bitterness, aftertaste and deliciousness of fructosylstevioside were greatly improved over those of stevioside.
