COMPARATIVE STUDY OF RANDOM AMPLIFIED POLYMORPHIC DNA OF TRYPANOSOMA CRUZI ISOLATES FROM PARAGUAY

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The variation of Paraguayan Trypanosoma cruzi isolates was examined using the unweighted pair group method analysis (UPGMA) based on the resulting matrix of the Dice similarity coefficients of random amplified polymorphic DNA (RAPD) profiles. These isolates were also compared with the established strains isolated from Brazil, Chile and Colombia in South America. Of 9 Paraguayan isolates, 3 isolates were similar to the Berenice, Y and Sao Felipe strains (zymodeme type; Brazilian Z2), respectively, isolated from Brazil, and 4 strains were similar to the Tulahuen strain from Chile. There was no isolate similar to the Colombiana I strain (Z1) from Colombia.