抄録
Biointerface composed of bimolecules with pH response, bioaffinity and protein immobilization functionalities using synthesized surface initiator coated on magnetic nanoparticles surface was successfully prepared by atom transfer radical polymerization (ATRP) in methanol at ambient temperature. The pH response polymer brush with 2-(diethylamino)ethyl methacrylate (DEA)-grafted magnetic nanoparticles can be dispersed in below pKa 7.3 aqueous solution due to protonation overcome magnetic interaction. On the other hand, polymer and block copolymer with bioaffinity and proteine immobilization functionalities based on 2-methacryloyloxyethyl phosphorylcholine (MPC)-grafted nanoparticles can be dispersed in neutral aqueous solution in spite of having a neutral charge. It is considered that steric repulsion of surface polymer brush has influenced the dispersibility. In the case of block copolymer composed of bioaffinity and protein immobilization functionalities, when the rate of immobilization of bovine serum albumin (BSA) was estimated, it turned out that the original protein immobilization part of surface polymer was exchanged for BSA. It is concluded that biointerface with various functionalities can be prepared by ATRP method to be useful for such as bioaffinity beads construction.
