抄録
The hydroxamate-typed artificial siderophore, tris[2-{3-(N-acetyl-N-hydroxamino)propylamido}propyl]aminomethane (TAPPA) was prepared and its FeIII complex, FeIII-TAPPA was modified on Au electrode surface. FeIII-TAPPA indicated biological activity for Microbacterium flavescens, which is hydroxamate-typed siderophore auxotrophic gram-positive microorganism, suggesting that FeIII-TAPPA was able to permeate the cell membrane of microorganism. The modification of Fem-siderophore complex was carried out by stepwise self-assembling method. The cyclic voltammetry of the resultant Au electrode, FeIII-TAPPA/Au confirmed the surface modification of Fem-TAPPA. The adsorption experiments of M. flavescens with FeIII-TAPPA/Au were clearly showed that FIII-TAPPA/Au could immobilize microorganisms. The images of the adsorption of M flavescens were obtained by various microscopic methods. Quart crystal microbalance (QCM) measurements also suggested that FeIII-TAPPA/Au was able to adsorb M. flavescens. The adsorption ability is due to the interaction between FeIII-TAPPA on a Au electrode and receptor/binding protein in the cell membrane.
