Polysaccharide composition of Ecklonia stolonifera Okamura and their enzymatic hydrolysis

抄録

Tsuruarame (Ecklonia stolonifera Okamura) belongs to Laminariaceae which is representative of brown algae. Tsuruarame is removed from sea area of Oma, Aomori Prefecture because of a bad influence on the production of Makonbu (Laminaria japonica Areschoug). In order to obtain the fundamental information for effective use of the removed Tsumarame, the polysaccharide composition of Tsumarame and their enzymatic hydrolysis were investigated. Sugar composition analysis suggested that laminaran, fucoidan, and cellulose were the types of polysaccharides present in the cell-walls of Tsuruarame, and that the carbohydrates in Tsuruarame and Makonbu had features in common. Commercial enzymes for the effective saccharification of Tsumarame polysaccharides were screened for. Meicellase P was selected. The amount of glucose released from the 4% KOH-pretreated, triturated Tsumarame by treatment with Meicellase P accounted for about 40-50 % of glucan in Tsumarame