Determination of Insulin Antbody by Use of Immunoadsorbent Latex

Abstract

A Simple and accurate method for the determination of insulin antibody was elaborated. In the technique small and stable polyazostyrene-insulin particles could agglutinate with insulin antibody with high sensitivity. The method has an advantage in its reproducibility, because the polystyrene-insulin latex can be preparedwith the constant amount of insulin as antigen and can be stored under 0°C at least six monthes. It was particulary suitable to determine the antibody to a minor molecular peptide-hormone, insulin.