Abstract
Our previous study showed that maltose did not stimulate insulin secretion from the Perfused rat pancreas for 30 minutes. However, slow effect of maltose on insulin secretion is expected, when maltose cleaves glucose in the B-cells of the pancreas. Male Wistar rats (200-250 g) were fasted for 20 hours. Preparation of the isolated pancreas was performed according to the procedure of Grodsky with a small modification. Krebs-Ringer bicarbonate buffer containing dextran (4.5 %) was used as perfusate.
In this experimental system, biphasic increase in insulin secretion was obtained by glucose (300 mg/100 ml), i. e. rapid secretion of insulin at 3 minutes and slow and sluggish increase thereafter. On the contrary an increase in insulin secretion appeared at 30 minutes after maltose (300 mg/100 ml) perfusion and continued until 90 minutes. This result shows that maltose stimulates insulin secretion without early rapid phase as seen in glucose perfusion.
