Precipitin Reactions between Monocomponent Insulin and Guinea Pig Anti-bovine Insulin Sera

Abstract

Five male guinea pigs were immunized by subcutaneous injections of bovine crystalline insulin emulsified in Freund's complete adjuvant. The guinea pig anti-bovine insulin sera (GPABIS) were kept at-20°C until used.
The experiment was carried out by using the multiple well double diffusion technique. Phosphate buffer saline (0.05M, pH 7.2) was used for the preparation of a 1.0% agar gel.
GPABIS produced a precipitin line with porcine as well as with bovine insulin. Monocomponent pork insulin also produced a precipitin line. The bovine and porcines precipitin lines were fused completely with the monocomponent precipitin line.
It was demonstrated that GPABIS contained precipitating insulin antibodies and produced precipitation with monocomponent insulin.