Abstract
The duration of the hypoglycemic effect of Monocomponent insulin (MI) was examinedcompared with conventional insulin (CI) in normal rabbits and rabbits immunized with beef insulin.
Using neutral soluble insulin preparations, there was no differrence for the maximal rate and duration of the hypoglycemic effect between the MI and the CI in the normal and immunized rabbits, but in the immunized rabbits the hypoglycemic effect of both insulin preparations was delayed as compared with normal rabbits.
On the other hand, using insulin zinc suspension preparations, in the normal rabbits, the maximal hypoglycemic response to MI was almost the same as that of CI, but the duration of the effect was shorter with MI than with CI. In immunized rabbits, the action of MI was slightly delayed. The maximal hypoglycemic response to the CI injection was apparently decreased and its effect continued weakly for a long time.
It was assumed that these phenomena observed with the insulin zinc suspensions were due to the different origins of the MI (made of pork crystalline insulin 70 and pork amorphous insulin30) and the CI (made of beef crystalline 70 and pork amorphous 30), and that the delay of insulin effect in the immunized rabbits was caused by weak binding of the pork insulin to the antibeef insulin antibody.
