Abstract
Rabbits were immunized with a-, b-component of bovine insulin, a-component of porcine, Insulin, conventional insulin or monocomponent insulin in order to compare the immunogenicity of these components.
Rabbits immunized with a-component of bovine insulin showed the strongest antibody production in early stage. All rabbits immunized with conventional insulin produced insulin antibodies. On the other hand, 2 out of 5 rabbits immunized with monocomponent insulin produced no antibody. Antibody developed against conventional insulin tended to be stronger than that against monocompo. nent insulin.
Rabbits immunized with b-component of bovine insulin showed glucose intolerane in intravenous glucose tolerance test, but these with a-component of bovine insulin showed no glucose intolerance and exhibited reactive hypoglycemia after glucose load.
It is suggested that a-component of bovine insulin may be most immunogenic, but the antibody developed against it may not affect on glucose tolerance.
