Abstract
Crystalline cholesterol was administered at 750 mg/day for 2 weeks to 23 well-controlled patients with non-insulin dependent diabetes mellitus (NIDDM).The changes of plasma lipoproteins in response to cholesterol load were compared with previously reported results in control (N= 23), aged (N=-18) and ischemic heart disease (IHD, N=15) groups.
VLDL was separated by ultracentrifugation and HDL by heparin-Mn 4 precipitation.The HDL fraction collected by heparin-Mn++ precipitation was dialyzed against NaCl solution of density 1.125, and the HDL2 fraction was then separated from HDL3 by ultra centrifugation.The LDL cholesterol value was obtained by subtraction of the VLDL and HDL cholesterol values from the plasma total cholestrol.
In NIDDM, the plasma VLDL, LDL, HDL, HDL2, and HDL3 cholesterol levels were not found to undergo significant changes on cholesterol load because of the wide variations.It was considered that a significant change had occurred when the LDL and HDL cholesterol levels were altered on 2 weeks' cholesterol load by more than 10% of the preloading value, and the trends of the changes in LDL and HDL cholesterol levels on cholesterol load were compared among the 4 groups.
The plasma LDL cholesterol levels were increased in 35% of the control, 6 % of the aged, 33 % of the IHD and 17% of the NIDDM groups, and decreased in 17% of the control, 63% of the aged, 14% of the IHD and 9% of the NIDDM groups on cholesterol load.The HDL cholesterol levels were increased in 52% of the control, 12% of the aged, 7% of the IHD and 35% of the NIDDM groups, and decreased in 13% of the control, 44% of the aged, 27% of the IHD and 14 % of the NIDDM groups.Based on these figures, the trends of changes in the LDL-C/HDL-C ratio on cholesterol load were compared.The LDL-C/HDL-C ratio was increased in 35% of the control, 17% of the aged, 53% of the IHD and 17% of the NIDDM groups on cholesterol load.
