Abstract
Remnant Like Particles (RLP) constitute a lipoprotein unbound to immunoaffinity gel mixture of and apo A-I and apo B-100 monoclonal antibodies coupled with Sepharose 4B. As already reported, RLP could thus be separated simply by a rapid method. It has also been reported that this RLP contains a chylomicron remnant and a very low density lipoprotein remnant. The concentrations of serum RLP-cholesterol (RLP-C) and RLP-triglyceride (RLP-TG) were determined in 217 non-insulin dependent diabetes mellitus (NIDDM: 62.3±9.8 years old) and 317 non-diabetic subjects (non-DM: 60.5±8.8 years old). The level of RLP-C was significantly higher in NIDDM (4.8+5.9mg/dl: mean±SD) than in non-DM (3.1±4.5mg/dl)(p<0.001). The percentage of subjects whose RLP-C concentration exceeded 5mg/dl was significantly higher in NIDDM (31%) than in non-DM (10%)(p<0.001). Even in the two groups of normolipidemic NIDDM and non-DM, the difference was signficant (p<0.001; 11%[15/136] in NIDDM and 3%[6/191] in non-DM, respectively). On the ather hand, the level of RLP-TG not differ significantly between NIDDM (34.5±31.2mg/dl) and non-DM (317±33.1mg/dl).
In NIDDM, RLP-C was lower in the good (fasting plasma glucose<140mg/dl and HbA1c<7.5%) than in the poor glycemic control group. RLP-C was further significantly augmented in macroalbuminuric patients (more than 200mg/g·creatinine, RLP-C: 7.8±11.4mg/dl) as compared tonormoalbuminuric (less than 10mg/g·creatinine, 4.2±4.2mg/dl) and microalbuminuric patients (10-200mg/g·creatinine, 4.4±4.7mg/dl). Thus, RLP-C was often elevated in NIDDM, even in patients with normolipidemia, and this elevation was dependent on glycemic control and an increased urinary albumin excreation rate.
In conclusion, RLP-C, which is a major component of cholesterol in apo-E rich remnant substance (lipoprotein remnant), was found to often be abnormally high in NIDDM, Thus atherosclerosis might be accelerated.
