LECTIN HISTOCHEMICAL STUDY OF THE KIDNEY IN UNINEPHRECTOMIZED AND DIABETIC (UN-D) MICE

Abstract

Lectin histochemical characteristics of the kidney of experimentally induced diabetic mice were investigated. Mice were divided into the following 4 groups: control (C), streptozotocin (SZ)induced diabetes (D), unilateral nephrectomy (UN), and unilateral nephrectomy-SZ-induced diabetes (UN-D) groups, and killed at 12 weeks after completion of the SZ-injection. No lectin histochemical differences were observed in the glomeruli of all groups. Stainabilities of Bowman's capsules lined with cuboidal to columnar epithelia seen in C and UN groups were the same as those of proximal tubules, and they were different from those of Bowman's capsules lined with flattened epithelia generally seen in D and UN-D groups. In D and UN-D groups, distal tubules and collecting ducts increased their affinities to Canavalia ensiformis A, Dolichos bifiorus agglutinin, Griffonia simplicifolia agglutinin-I, Glycine max agglutinin, and Triticum vulgaris agglutinin, compared with C and UN groups. Conclusively, this study showed that unilateral nephrectomy enhanced SZ-induced kidney lesions without a prominent histochemical modification.