微小電極アレイシステムによる痙攣・てんかん評価法確立の試み（第5報）―CSAHi 神経チーム

白川 誉史; 宮本 憲優; 石橋 勇人; 佐藤 薫; 相川 信夫; 小島 敦子; 小田原 あおい; 柏崎 広美; 小山 隆志; 阿部 浩幸; 佐治 大介; 田村 麻耶; 加藤 正巳; パブラック 昌子; 島戸 大来; 申 義庚; 森村 馨; 鈴木 郁郎

doi:10.14869/toxpt.47.1.0_P-134

The 47th Annual Meeting of the Japanese Society of Toxicology

Session ID : P-134

DOI https://doi.org/10.14869/toxpt.47.1.0_P-134

Conference information

Host: The Japanese Society of Toxicology

Name : The 47th Annual Meeting of the Japanese Society of Toxicology

Date : 2020 -

Poster

An attempt to establish the evaluation system for drug-induced epilepsy by applying microelectrode array recording -activity report from CSAHi Neuro group

*Takafumi SHIRAKAWA, Norimasa MIYAMOTO, Yuto ISHIBASHI, Kaoru SATO, Nobuo AIKAWA, Atsuko OJIMA, Aoi ODAWARA, Hiromi KASHIWAZAKI, Takashi KOYAMA, Hiroyuki ABE, Daisuke SAJI, Maya TAMURA, Masami KATO, Akiko PAWLAK, Daiki SHIMATO, Wigyon SHIN, Kaoru MORIMURA, Ikuro SUZUKI

Author information

*Takafumi SHIRAKAWA
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Astellas Pharma Inc.
Norimasa MIYAMOTO
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Eisai Co., Ltd.
Yuto ISHIBASHI
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Tohoku Institute of Technology
Kaoru SATO
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Laboratory of Neuropharmacology, Division of Pharmacology, National Institute of Health Sciences
Nobuo AIKAWA
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Kyowa Kirin Co., Ltd.
Atsuko OJIMA
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Eisai Co., Ltd.
TechnoPro, Inc. TechnoPro R&D, Company
Aoi ODAWARA
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Tohoku Institute of Technology
Hiromi KASHIWAZAKI
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Otsuka Pharmaceutical Co., Ltd.
Takashi KOYAMA
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Otsuka Pharmaceutical Co., Ltd.
Hiroyuki ABE
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
NISSEI BILIS CO., LTD.
Daisuke SAJI
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
NISSEI BILIS CO., LTD.
Maya TAMURA
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Axcelead Drug Discovery Partners Inc.
Masami KATO
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Nihon Bioresearch Inc.
Akiko PAWLAK
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Nihon Bioresearch Inc.
Daiki SHIMATO
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Nihon Bioresearch Inc.
Wigyon SHIN
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
FUJIFILM Corporation
Kaoru MORIMURA
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
FUJIFILM Corporation
Ikuro SUZUKI
Consortium for Safety Assessment using Human iPS Cells (CSAHi)
Tohoku Institute of Technology

CONFERENCE PROCEEDINGS FREE ACCESS

Details

Abstract

Our CSAHi neuron team aims to develop in vitro system using a micro-electrode array (MEA) for detection of seizure. We investigated the versatility of MEA systems using two types of rat neuron cells under multi facilities. Gabazine, Picrotoxin (PTX), 4-AP and Acetoaminophen (APAP) were used. As a result, in all facilities, Gabazine and PTX could be separated from APAP in two samples and in two MEA systems, but not 4-AP in facilities where the number of spikes and firing bursts increased in APAP. MEA is a versatile evaluation system, and the responses to the negative compounds may be important.

Corresponding author

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