Comparison of acute inhalation toxicity of sulfuric acid between intratracheal instillation and whole-body inhalation

Abstract

Intratracheal instillation (ITI) method is attracting attention as an alternative for aerosol inhalation toxicity test. In this study, the acute inhalation toxicity of sulfuric acid between ITI and whole-body inhalation was compared. Sulfuric acid was administered to rats by ITI. General condition and body weight were evaluated until 14 days after administration, and then pathological examination were conducted. LD₅₀ of sulfuric acid by ITI was estimated to be 7 to 20 mg/kg. The acute inhalation toxicity by ITI was considered to more than twice as strong as that by whole-body inhalation.