Gelatin fiber scaffold is a strong tool for evaluation of drug response in iPSC derived cardiomyocytes

Abstract

To predict human cardiac adverse effects in vitro, electrophysiological studies and calcium imaging with human induced pluripotent stem cells derived cardiomyocyte (hiPSC-CM) has been used. However, these systems generally use rigid cell- culture dish, dynamic motions are suppressed. The gelatin hydrogel fibrous nonwoven (GHFN) has both flexibility and deformation- recovery property. These nature are expected to give dynamic and stable motion of CM. We achieved simultaneous measurement of calcium transient and dynamic motion from hiPSC-CM. Drug response of iPSC-CM on GHFN will be reported.