新鮮ヒト肝細胞（PXB-cells®）を用いた薬剤性脂質症評価

山尾 美香留; 稲松 睦; 喜早 慧士; 小川 裕子; 山崎 ちひろ; 石田 雄二; 立野 知世

doi:10.14869/toxpt.47.1.0_P-227

The 47th Annual Meeting of the Japanese Society of Toxicology

Session ID : P-227

DOI https://doi.org/10.14869/toxpt.47.1.0_P-227

Conference information

Host: The Japanese Society of Toxicology

Name : The 47th Annual Meeting of the Japanese Society of Toxicology

Date : 2020 -

Poster

Development of an assay for drug-induced steatosis using fresh human hepatocytes (PXB-cells^®)

*Mikaru YAMAO, Mutsumi INAMATSU, Keishi KISOH, Yuko OGAWA, Chihiro YAMASAKI, Yuji ISHIDA, Chise TATENO

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Abstract

We previously showed that human hepatocytes (PXB-cells^®) freshly isolated from chimeric mice with humanized livers (PXB-mice^®) could be maintained expressing major hepatic genes up to 3 weeks.

In this study, we evaluated whether an in vitro model using PXB-cells can be used to predict the risk of drug-induced steatosis. PXB-cells were treated with valproic acid (VPA) in a medium supplemented with fatty acids. VPA induced lipid accumulation in PXB-cells at 3 days after treatment. It was suggested that PXB-cells would be useful in predicting the risk of drug-induced steatosis.

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