Effect of blood microsampling method on evaluation of a non-clinical safety study in rats–Oral gavage administration of phenacetin for 4 weeks–

Abstract

Blood microsampling (MS) was introduced in rats treated with phenacetin for 4 weeks and examined whether MS affect the toxicological parameters induced by phenacetin. The findings indicative of hemolytic anemia were observed in hematological parameters and histopathology in the liver, spleen and bone marrow both in rats with and without MS except for the hematocrit value, which was decreased only in rats without MS. In conclusion, MS had limited effect on the toxicological parameters induced by phenacetin and satellite animals for toxicokinetics could be eliminated in the toxicity study.