Optimization of assessment method for drug induced contractile response in human induced pluripotent stem cell-derived cardiac cell sheet-tissue

Abstract

Isometric contractile force was recorded using cell-sheet-tissues. The drug effects were evaluated at 3 different electrical stimulation conditions as follows; spontaneous beat, 1 Hz pacing, and spontaneous beat after preconditioning (72hr stimulation at 2 Hz for maturation purpose). Drugs which were difficult to measure under spontaneous beat could be evaluated under 1 Hz pacing without beating arrest, or exhibited large increase of contractile force after preconditioning. Taken together, this method can be a very useful tool for preclinical study.