Accuracy control of Percellome Toxicogenomics data and its influence on the data analysis

Abstract

The basis of the data accuracy control for the Percellome Project was established when the Toxicogenomics Project (NIBIOHN) was initiated in 2003 to accumulate rodent gene expression data for over 150 chemicals. We checked the dose-response performance of the microarrays and developed a RNA spiking method and related softwares to generate absolutized copy number of mRNAs per cell. Accuracy control is essential for the newly designed “repeated dosing studies” which monitors a genome-wide alteration in basal gene expression levels. (supported by Health and Labor Sciences Research Grant)