Host: The Japanese Society of Toxicology
Name : The 47th Annual Meeting of the Japanese Society of Toxicology
Date : 2020 -
Research and development of nucleic acid drugs and genome editing drugs using CRISPR-Cas9 system have been progressing. Although these modalities are designed to have high selectivity to target sequence, they can also bind to non-target genes having homologous sequences even if the sequences are not completely homologous. This is commonly referred to as the hybridization-dependent off-target effect.
This presentation will focus on in silico analysis on off target effects of these modalities. I would like to show examples of an initial evaluation of antisense oligonucleotides and guide RNA of CRISPR-Cas9 and discuss methodologies and difficulties from the perspective of nonclinical toxicologist.
