Evaluation of new in vitro screening system to detect drug-induced liver injury by using new culture plate with low drug adsorption and high oxygen permeability

Abstract

The potency of mitochondrial dysfunction and BSEP inhibition is an useful factor to predict liver injury in the preclinical stage. However, hepatocellular functions are rapidly decreased using polystyrene plate, which is the lack of oxygen supply. So, conventional method is difficult to detect the risk of liver injury adequately. We newly developed an oxygen-permeable plate with low drug adsorption, called “T plate”. So, we evaluated hepatocellular function and the sensitivity of cell death in primary rat hepatocyte cultured by T plate.