Characteristics of small intestinal epithelial cells derived from human induced pluripotent stem cells and usefulness as an in vitro model for gastrointestinal toxicity evaluation

Abstract

F-hiSIEC^TM is a human iPS cell-derived small intestinal epithelial-like cell which shows the physiological characteristics similar to those of the in vivo human small intestine such as CYP3A4 activity and expression of transporter genes. To extend the applicability of F-hiSIEC^TM to gastrointestinal (GI) toxicity assessment, several types of reference drugs were examined in this study. The results of the present study show an in vitro system using F-hiSIEC^TM can be a useful model for evaluating GI toxic agents and effects of drug candidates on differentiation of intestinal epithelium.