Construction of Hepatic Vascular Model and Toxicity Assessment System that Can Predict DILI Compounds

Abstract

We constructed a hepatic vascular model for predicting hepatotoxicity by using our unique tissue-engineering method. Briefly, we created a viscous body by suspending cells with heparin and collagen. The viscosity can induce cell-cell adhesion suitable for constructing a hepatic tissue. We constructed the vascularized tissue whose hepatocytes ratio is 65% (close to in vivo). We confirmed the predictive performance of DILI by treating typical DILI compounds. Notably, we could predict the toxicity of Monocrotaline by vessel analysis with higher sensitivity than usual viability assay.