Detection of thalidomide in seminal plasma after oral doses in male rabbits

Abstract

A developmental toxicity test system for intravaginal exposures via semen should be newly considered. The teratogenicity of chemotherapeutic drug thalidomide is species-specific in primates and rabbits. Seminal plasma concentrations of thalidomide and its 5‑hydroxylated metabolite were detected after oral doses (2, 250, and 500 mg/kg) of thalidomide in male rabbits (NZW) by liquid chromatography-mass spectrometry, in a similar time-dependent manner to the blood plasma. Based on this toxicokinetic information, an intravaginal administration of thalidomide to female rabbits will be conducted.