Development of signal pathway analysis method utilizing intracellular localization information of protein

Abstract

Nikon Corporation, in collaboration with research group of Professor Masayuki Murata*, Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, has developed a new signal pathway analysis method that estimates and visualizes the activated signal pathway from immunostaining images of dozens of proteins utilizing intracellular localization information of proteins.

In this method, at first the proteins are quantified where, when, and how the protein behaves in the cell.

Next, by calculating the correlations of those quantified data, the relationship between proteins including intracellular localization information is estimates and visualized.

This make it possible to capture changes in the signal pathway due to drugs.

In this presentation, we will introduce an example of applying this method to insulin response research.

*Currently, “Multimodal Cell Analysis Collaborative Research Cluster, Institute of Innovative Research, Tokyo Institute of Technology”, “Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology”, “International Research Center for Neurointelligence, Institutes for Advanced Study, The University of Tokyo”.