Host: The Japanese Society of Toxicology
Name : The 51st Annual Meeting of the Japanese Society of Toxicology
Date : July 03, 2024 - July 05, 2024
Currently, carcinogenesis studies in rodents require a long period of time. Even using genetically modified animals, the administration period is about 26 weeks. Therefore, the problems of labor and cost have been pointed out. Organoids are widely used in human and animal cancer research because they can reproduce in vivo epithelial tissue structures and gene expression patterns in culture dishes. Although the usefulness of carcinogenesis models using organoids derived from normal mouse tissues (lung, liver and mammary gland) has been reported in previous studies, there are no reports of carcinogenesis models using organoids derived from normal bladder tissues. In this study, we attempted to create a carcinogenic organoid model using normal rat bladder tissue. Organoids were prepared using bladder tissue from 6-week-old F344/DuCrlCrlj rats, treated with 2-Acetylaminofluorene (2-AAF) for 6 days, and then transplanted into immunodeficient mice, resulting in tumor formation 7 days after implantation. On 21 days after transplantation, the tumor tissue removed and confiemed the expression of bladder epithelial cell markers (CK5 and CK7) and histopathological features. Subsequently, organoids were prepared from tumor tissue and transplanted into immunocompromised mice, resulting in tumor re-formation. Tumor tissue removed after the second organoid implantation was histopathologically confirmed to be squamous cell carcinoma.
