Host: The Japanese Society of Toxicology
Name : The 51st Annual Meeting of the Japanese Society of Toxicology
Date : July 03, 2024 - July 05, 2024
Proteins are the end products of genes and are molecules with a variety of functions. These changes cause a wide variety of biological phenomena. Therefore, it is essential to measure proteins to understand the phenomena that occur in vivo. Protein expression levels are best measured on the protein itself by proteome analysis, but are frequently predicted by transcriptome analysis. The reason for this is the small number of proteins that can be observed in a simple proteome analysis. However, recent studies have shown that the correlation between protein and mRNA expression levels is not high, which inevitably increases the significance of measuring the protein itself. Therefore, there is a need to further improve the simplicity and depth of proteome analysis. In 2018, our laboratory has started to develop a proteome system that can detect all proteins expressed in cells in a single-shot analysis without fractionation. Focusing on data independent acquisition (DIA)-MS technology, we were able to detect 10,000 proteins from HEK293 cell digests in 2022 (Kawashima Y. et al., J. Proteome Res., 21, 2022). Furthermore, by combining a state-of-the-art MS and a 60 cm long column, we succeeded in detecting 13,000 proteins, which are almost all proteins contained in HEK293 cells. This ultra-deep proteome analysis system will be very useful in the search for master factors for the effects of drugs and metabolites on cells and tissues.
