Effects of chondroitin sulfate B on the reconstitution of collagen fibrils

Abstract

Collagen fibrils were reconstituted in vitro from type I collagen solution. The reconstituted collagen fibrils and chondroitin sulfate B were deposited alternately. The adsorbed weight of the collagen fibrils and chondroitin sulfate B was measured using a quartz crystal microbalance (QCM) and their microstructure was observed using an atomic force microscope (AFM). The adsorbed weight of the collagen fibrils on the adsorption of collagen fibrils and chondroitin sulfate B alternately was significantly larger than that on the adsorption of collagen fibrils only. The adsorption of chondroitin sulfate B was observed on the surfaces of collagen fibrils. These results indicate that chondroitin sulfate B is effective on the reconstitution of collagen fibrils.