This paper proposes the fluorescence response-based optical probing (FROP) method for the 3-dimensional measurement of precise products. Several 3-dimensional measurement methods exist, such as micro-coordinate measuring machines, confocal microscopy, and point autofocus microscopy. However, measuring precise products with small, smooth, and steep (3S) structures—such as die molds and optical lenses—remains challenging. In this study, we propose a new surface detection scheme that utilizes autofluorescence from the sample surface. Unlike reflected light, fluorescence is emitted over a wide angle. Therefore, the optical response from the surfaces of 3S structures can be obtained by exciting fluorescence at the measured surfaces. This paper first explained the principle of FROP. Next, the fundamental FROP signal was examined on surfaces tilted at different angles. The FROP successfully detected vertical and even overhanging surfaces, demonstrating its strong potential for 3-dimensional measurement. The principle of surface position determination was then verified through comparisons with conventional confocal microscopy for 2.5D measurements, and thickness measurement results were compared with micrometer results. These results revealed that the peak position of the differentiation signal in FROP coincided with the sample surface. Finally, a 3-dimensional 3S structure was measured. The results confirmed that vertical surfaces could be successfully measured using the FROP method, whereas conventional confocal microscopy could not measure them. Consequently, the performance of FROP for 3-dimensional measurement of precise products was validated.
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