Effect of shear stress on orientation and migration of myoblast

Abstract

Mechanical stimulation affects cell behaviors (proliferation, orientation, migration and differentiation) in vivo, and a lot of models of experiments on mechanical stimulation in vitro have been reported. The development of control technique on cell is important in the field of regenerative medicine. Recently, skeletal myoblasts have been applied for cardiac repair. In the previous study, C2C12 (mouse myoblast cell line) made orientation perpendicular to the streamlines in the donut shape flow channel. In the present study, C2C12 has been cultured in the Couette type of the shear field between the rotating disk and the stationary culture plate to study quantitatively the effect of shear stress (for 24 hours, < 2 Pa) on orientation of myoblasts in vitro. The time lapse image of myoblasts shows that C2C12 tilts perpendicularly against the flow direction at the wall shear stress of 2 Pa and that C2C12 tends to migrate to the lower wall shear stress region of 0.4 Pa.