2011 Volume 40 Issue 1 Pages 12-17
Identification of anthocyanins and a simple method for their content measurment in bentgrass were established in this experiment. 1) Two anthocyanins, cyanidin 3-glucoside and cyanidin 3-malonylglucoside were identified in the leaves of bentgrass by chromatography with authentic anthocyanins. 2) The most suitable solvent for extraction of anthocyanin was 3% aqueous HCL. 3) A simple method for anthocyanin content measurment in bentgrass was established as follows: After homogenizing of leaf sample (0.2 g FW) with 20 ml of 3% aqueous HCL, the sample is kept for 24 hours under dark conditions. After filtration with paper, the sample solution is diluted volumetrically to appropriate concentration and the absorbance of extraction is measured at 530 nm. 4) Seasonal change in anthocyanin content of the anthocyanin accumulative strain was low from spring to summer and high from late autumn to winter. While, that of the non-accumulative strain was low in winter season and almost same level from spring to early autumn of the accumulative strain.
