Abstract
Thiamine metabolism was followed in developing chick embryos. There is no change in the total thiamine content during the entire course, but with development of the embryo, thiamine is transferred to the body of the chick and it is in the bound form. With development of the amnion and yolk sac, there is a gradual decrease in the free form, but the thiamine present in the amnion and amnionic fluid is for the greater part, the free form. The thiamine content of the chick increases with development and is found in the greatest quantities in the brain and muscles. The other organs in the order of their thiamine content are the heart, liver and intestines.