The destruction products of thiamine by Bacillus aneurinolyticus cell suspension were isolated by paper column chromatography : the one was 4-methyl-5-β-hydroxyethylthiazole [yield : 63% as the picrate (mp. 160-161℃)], and the other was 2-methyl-4-amino-5-hydroxymethylpyrimidine [yield : 41% as the free base (mp. 190-194℃), 30% as the HCl-salt (mp. 219℃ (decomp.)].
