Abstract
As a preliminary sudy for the assay of folic acid and citrovorum factor in human urine by the use of Streptococcus faecalis and Leuconostoc citrovorum, the relationship of these bacteria to some salts in urine was examined. It was found that oxalate inhibits the growth of S.faecalis and Lc.citrovorum when added to the assay medium in excess of 5 mg per 10 ml of medium. The folic acid and citrovorum factor contents of urine were not increased by digestion with enzyme solutions prepared from hog kidney or chicken pancreas, indicating that there were no combined forms of folic acid and citrovorum factor excreted in urine. The urinary excretion levels of folic acid and citrovorum factor varied from 5.2 to 1.8 μg (3.7 μg on an averge), and from 1.4 to 0.5 μg (0.8 μg), respectively per bay in normal adults.
