Abstract
The urinary excretion of biotin before and after biotin administration and the biotin content of liver were studied in rabbits with acute or chronic CCl_4 damage. In acute CCl_4 poisoning the urinary excretion of biotin was found to be increased even on the first day. The high level continued until the second day, and decreased slightly on the third day. In the chronic CCl_4 poisoning, the spontaneous excretion of biotin and the increase of urinary excretion after an administration of 40μg of biotin increased gradually. At the end of eighth week, the increase in these values were significant. The biotin content of liver, especially the bound biotin, was found to be decreased remarkably, both in acute and chronic CCl_4 poisoning. These facts afford an additional evidence to the hypothesis that there is disturbance of utilization of biotin in hepatic damage.
