Abstract
OMP, a toxic decomposition product in thiamine injection was fixed chromatographically according to the method presented in the previous paper. From examination of 51 injections with varying shelf life ranging from 1 to 10 years, it was presumed that older preparations may contain OMP to a considerable amount. OMP which was fixed on filter paper from 12 representative injections was eluted with the aid of dilute hydrochloric acid (PH 1.0) and then determined spectrophotometrically. It's absolute content in injection extended from 0.02 to 1.27mg per 1ml of solution and these figures were equivalent to 0.2-3.8mg of OMP per 100mg of thiamine or decomposition of 0.5-9.2% of originally prescribed thiamine. A certain parallelism was found between decrease in thiamine and increase in OMP.
