A SIMPLIFIED PREPARATION METHOD OF THE P^<32>-LABELED FMN AND FAD

Abstract

The P^<32>-labeled FAD was prepared from rabbit liver which was injected with a certain dose of P^<32>-phosphate and FMN. The extraction was made by Crammer's method, and the crude preparation was further purified by two phase chromatography (developers : 5% citrate・isoamylalcohol). P<32>-labeled FMN was obtained by hydrolysing FAD obtaining 5% trichloroacetic acid, and purified by the above solvent. In the present paper, the preparation method of P^<32>-labeled FAD and FMN, and the fate of these compounds in rats injected with them were deal with.