Abstract
It was found that thiochrome was produced by the addition of phosphomolybdic acid followed by NaOH to thiamine solution. The finding led to the investigation on the optimum condition for this reaction. Maximum quantity of thiochrome was obtained by the reaction of 5 ml of thiamine solution with 10 mg of phosphomolybdic acid at pH 8 at 60℃ for 5 minutes. The yield of thiochrome compares with that with any of potassium ferricyanide or cyanogen bromide.
