Abstract
Investigations on the chromatographic developing agents for riboflavin and its derivatives showed that a mixture of n-butanol・acetic acid・water (4 : 1 : 5) was suitable for separation of the vitamins, and the use of an aqueous solution of 5% Na_2HPO_4 enabled the elimination of interfering substances for the estimation of riboflavin in the urine since the Rf value of the substances became larger than those of the vitamins. For paper chromatographic determination of three forms of riboflavin, it is necessary to employ two dimensional procedure using n-butanol・acetic acid・water as a primary solvent and 5% Na_2HPO_4 as a secondary one. High riboflavin concentration and low interfering fluorescence in the urine of healthy infants fed with riboflavin-enriched powdered milk enabled the separation from interfering substances and estimation of riboflavin, while in case of normal children without supplement of riboflavin the fluorometric estimation of the vitamin was difficult due to the low vitamin as well as high interfering fluorescence. No yellow fluorescent band corresponding to FAD could not be recognized in the present experiment.
