STUDIES ON BACTERIAL THIAMINASE : (I) COMPARATIVE STUDIES ON ANAEROBIC CULTURE METHOD FOR THE PRODUCTION OF THIAMINASE BY ANAEROBIC THIAMINE DEGRADING BACTERIA

Abstract

The relationship between the growth of bacteria and the produdtion of thiaminase was studied by culturing Clostridium thiaminolyticum anaerobically with various methods. In peptone water, it grew best with the double tube method, in which least oxygen was dissolved. In neutrient broth, it grew well in any method used, but the less oxygen was dissolved, the more thiaminase was produced. Thus the growth of the bacteria and the production of thiaminase was found to be dependent on the condition of culture.