Abstract
Thiamine-degrading bacteria have been isolated from canine feces collected in Osaka Prefecture. Five strains of Bacillus thiaminolyticus and fifteen strains of Clostridium thiaminolyticum were isolated from 87 canines. Comparative studies were made for isolating thiamine-degrading bacteria with both microbiological assay and chemical method and the usefulness of micrbiological method was ascertained.