It was observed that partly purified thiaminase II of Bacillus aneurinolyticus catalysed the formation of thiothiamine from 2-methyl-4-amino-5-hydroxymethylpyrimidine and 2-mercapto-4-methyl-5-β-hydroxyethylthiazole, and that thiothiamine could be used for the growth of Bacillus aneurinolyticus, instead of thiamine.
