Abstract
Thiamine contents and concentrations of livers, hearts, kidneys and intertines of rats on normal, thiamine-rich or thiamine-deficient diet were determined by thiochrome method after the animals had been perfused in situ with 0.85% saline solution through jugular veins. It was shown that thiamine concentrations of livers and intestines of both normally-fed and thiamine-rich rats had significant differences between nonperfused and perfused animals, whereas no significant differences between them were observed on thiamine-deficient rats. The percentage of phosphorylated thiamine to total thiamine contents of organs underwent no appreciable change after perfusion. The perfusion of isolated rat livers showed a great outflow of thiamine at the initial 30 minutes, then gradual decrease between 30〜120 minutes and still a small quantity of thiamine-outflow even after 120 minutes. Surface-active agents such as Igepon T, Span 60 or quarternary ammonium chloride, added to the saline solution at 20〜50 mg % concentrations on the last stage of ordinary perfusion had little effects to drain off further amounts of thiamine from livers.
