Abstract
Previous reports from our laboratory have shown that the bacterial cell fractions obtained from the supernatant liquor of night soil treatment plant and distillers' wastes digester have high microbiological activities for vitamin B_<12>. We were, however, afraid that these cell fractions might contain some vitamin B_<12> factor other than cyanocobalamin. This paper is devoted, therefore, to the paper electrophoretic examination of vitamin B_<12>-like compounds in the newly found resources. The results demonstrated that pseudovitamin B_<12>. Factor A, Factor B and a compound supposed to be Factor C are contaminated in the extract of cell fraction, but that 70〜80% of the total activity is due to cyanocobalamin and Factor III.